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There may be issues of data protection and patient con dentiality to be dealt with when obtaining ethical consent 34 Disaggregation and Primary Culture Detailed information on disaggregation as a method for obtaining cells is provided in the appropriate chapters Brie y, the tissue will go through stages of rinsing, dissection, and either mechanical disaggregation or enzymatic digestion in trypsin and/or collagenase It is often desirable not to have a complete single-cell suspension, and many primary cells survive better in small clusters Disaggregated tissue will contain a variety of different cell types, and it may be necessary to go through a separation technique [See 15, Freshney 2005], such as density gradient separation [Pretlow and Pretlow, 1989] or immunosorting by magnetizable beads (MACS), using a positive sort to select cells of interest [Carr et al.

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, 1999] or a negative sort to eliminate those that are not required [Saalbach et al, 1997], or by using uorescence-activated cell sorting (FACS) [See, eg Swope et al, 1997] The cell population can then be further enriched by selection of the correct medium (eg, keratinocyte growth medium (KGM) or MCDB 153 for keratinocytes [Peehl and Ham, 1980]), many of which are now available commercially (See Sources of Materials), and supplementing this with growth factors Survival and enrichment may be improved in some cases by coating the substrate with gelatin, collagen, laminin, or bronectin [Freshney, 2005]..

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Renewed interest in renaming SIOP to accurately re ect the scope of the science and practice recently materialized (i.e., Church, 2000). During the early years, de nitions described the eld almost exclusively as a technology with a focus on practical issues. For example, Kingsbury (1923) stated that business psychology or psychotechnology is interested in acquiring facts and principles only in so far as they can be turned directly to account in the solution of practical problems, in industry, selling, teaching, or other elds of human behavior (p. 5). Forty- ve years later, Blum and Naylor (1968) de ned the discipline as simply the application or extension of psychological facts and principles to the problems concerning

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positive than when the second born in the family was labeled as the gifted one That is, when the second born was labeled gifted, her or his sibling relationship with the older child was perceived to be less positive Such an interaction between birth order and identification of one child as talented could also occur in the athletic setting Another interesting and perhaps relevant study was conducted by Rust, Golombok, Hines, Johnston, Golding, and The ALSPAC Study Team (2000), who investigated the effects of having an older sibling on the degree to which preschoolers would exhibit gender-stereotypical behaviors The results of this observational study indicated that preschoolers ( both boys and girls) who had older brothers were more masculine and less feminine in their play behavior and general activity.

In contrast, preschool boys who had older sisters were more feminine but not less masculine, and preschool girls with older sisters were less masculine but not more feminine A similar, but retrospective, study conducted by Colley, Griffiths, Hugh, Landers, and Jaggli (1996) revealed that children and adolescents who had same-sex siblings showed more gender-stereotypical preferences in their play behavior than did those children who had opposite-sex siblings The results of these studies, as well as others (eg, Steegmiller, 1980; Stoneman, Brody, & MacKinnon, 1986; Sutton-Smith & Rosenberg, 1970), indicate that siblings do have an effect on each other with regard to the development (or nondevelopment) of gender-stereotypical attitudes, beliefs, and behaviors However, there are still discrepancies as to exactly how sibling influences operate in this area as well as how birth order, gender of siblings, and number of siblings might interact.

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